An Embryo Staging System for Comparison of Zygotic and Somatic Embryo Development
نویسندگان
چکیده
An embryo classification system has been developed based on observations of Pseudotsuga menziesii (Mirb.) Franco (Douglas-fir) and Pinus taeda L. (loblolly pine) zygotic embryos and on the literature concerning embryology within the pine family. This system is suitable for classification of both zygotic and somatic embryos of loblolly pine, Picea abies L. (Norway spruce), and Douglas-fir. This system can be used to compare early, mid, and late stages of zygotic and somatic embryos. The use of this system has been found to help in the formation of embryo development protocols for loblolly pine. In addition, this system has been useful in staging zygotic embryos for physiological and biochemical studies. Loblolly pine somatic embryos from liquid medium cell suspensions and from subsequent development on semisolid medium are compared to zygotic embryos.
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